Once the novel molecule is planted, preformulation study is one of the fractions that is started. In a larger sense, it covers with research on a molecule's physical, chemical, analytical, and medicinal properties and offers suggestions for effective modifications that could be made to improve performance. The study of preformulation factors can contribute to the development of pharmaceutical formulations that are dependable, safe, stable, and efficacious. Pantoprazole is an irreversible proton pump inhibitor (PPI) that reduces gastric acid secretion. PPIs, pantoprazole binds to the proton pump (H+,K+ -adenosine triphosphatase) in the parietal cells to exercise its pharmacodynamic effects; however, in comparison to other PPIs, its binding may be more specific for the proton pump. When given as an enteric-coated, delayed-release tablet, pantoprazole is well absorbed and has a 77 % oral bioavailability. It is metabolized by the liver's cytochrome P2C19 into the inactive metabolite hydroxypantoprazole, which is then subjected to sulphate conjugation. Independent of dose, the elimination half-life ranges from 0.9 to 1.9 hours. Similar to other PPIs, pantoprazole is effective in promoting the healing of gastric and duodenal ulcers. In the present works overall objective of preformulation studies of Pantoprazole is to engender information useful in developing stable and Bioavailable dosage forms.

Objectives: The benefits of topical drug delivery systems, which have been used for centuries, include the ability to deliver medications both quickly to the affected area where they are most effective and over an extended period of time. These systems lengthen the drug's mean resident time and contact time. The design and characterization of an antibiotic emulgel for topical medication administration is a goal of the current investigation. Methods: Cefpodoxime Proxetil emulgel were prepared using different concentration of Carbopol 934, HPMC K4M and xanthan gum as gelling agents and evaluated the relevant parameters such as physical examinations, pH, extrudability, spreadability, viscosity, swelling index, drug content, in-vitro diffusion studies and microbiology activities. Results: All formulations are neutral and viscosity of emulgel was found in the acceptable limits. On physical evaluations were found to be optimum in terms spreadability, swelling index and extrudability. Drug content of all formulations were found in the ranges 69.73% to 97.58% and CEF4 emulgel exhibiting the highest drug concentration and the lowest percentage drug release due to its controlled release pattern and proven non-fickian diffusion mechanism release. The results found that, the selected formulations proven better bacterial activities against both gram positive and gram negative organisms. Conclusions: Type and concentration of polymers can have an impact on the drug permeability studies and physical-chemical characteristics of the developed antibiotic emulgel, which had excellent results and was suitable for possible therapeutic purposes.

Infectious pathologic changes caused by pathogens, which are harmful that enter the body by bacteria, fungus, viruses, and parasites. Infectious-diseases can range in severity from very mild to very dangerous. Numerous human diseases are caused by bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris and Bacillus subtilis. Capparis spp. that have anti-bacterial, anti-oxidant and anti-inflammatory properties, including spermidine, carotenoids, quercetin, tocopherol, and rutin by the anti-bacterial, anti-fungal, and anti-leishmanial action. Carrot seed oil (Daucus carota) and tea tree oil (Melaleuca alternifolia) both exhibit antibacterial action respectively. Curcumin and its derivatives were found to have more potent anti-bacterial activity against several strains of S. pneumonia. Oregano oil and carvacrol has revealed that they have anti-viral properties against respiratory-syncytial-virus (RSV), that causes respiratory disease, rotavirus, a frequent condition of diarrhea in theng children, and herpes simplex virus type 1 (HSV-1). Sweet basil extract, which contain ingredients like ursolic acid and apigenin, have powerful antiviral properties against hepatitis B, herpes and enterovirus. Candida infections have fewer severe side effects and less cost load than chemical medications, can be treated with new pharmaceuticals, plant, and herbal items.

Background: Benign Prostate Hyperplasia (BPH) is an age associated prostate gland enlargement in men that can lead to urination difficulty. The exact etiology of BPH is unknown. However, inflammation is a critical factor in the development of BPH. Soursop as a traditional medicine has anti-inflammatory properties. This study investigated the anti-inflammatory potentials of soursop pulp extracts on induced benign prostatic hyperplasia (BPH) in adult male wistar rats. Materials and methods: Twenty-four adult male rats grouped into five were used for the study. BPH was induced subcutaneously in the inguinal region with testosterone propionate 3mg/kg/day. Acute toxicity test was carried out to determine the safety of the extract. The rats in group A served as the control group. Rats in group B, C and D were administered 200mg, 400mg, and 600mg of aqueous soursop extract per kg body weight/day. Biochemical analyses of the protein specific antigen, albumin, white blood cell count, testosterone, and C-reactive protein were performed after the trial. Statistical analysis was done using SPSS version 23. Results: Aqueous soursop pulp extract significantly decreased (p<0.05) the protein specific antigen by 58.87%. The extract at 400mg resulted in a percentage decrease in white blood cell count by 35.37% and albumin by 31.23 %. The extract at 600mg significantly decreased the testosterone and C- Reactive Protein by 59.88% and 69.47% respectively. Conclusion: The inflammatory bio-markers (prostate specific antigen, albumin, white blood cell count, testosterone, and C-reactive protein) studied after treatment revealed that aqueous soursop pulp extract is promising in the management of benign prostate hyperplasia.

Obesity is a major health hazard not only for developed countries but also for Bangladesh and it is an established risk factor of breast cancer. In our country there is a scarcity of studies on the associations of BMI and different features of breast tumour. This study was aimed to evaluate the associations of BMI with molecular sub-types, clinical and pathological characteristics of breast cancer in Bangladeshi women. This cross sectional descriptive type of observational study was conducted in National Institute of Cancer Research and Hospital (NICRH), Mohakhali, Dhaka from September, 2019 to August, 2020. A total of 90 patients with breast cancer were selected purposively according to inclusion and exclusion criteria. An informed consent was sought from the patient to take part is this study. Detail history taking thorough physical examination was done along with relevant investigations. Data were collected by semi structured questionnaire and analysis was done with the help of Statistical Package for Social Science (SPSS), version 21.0. Mean age of the respondents was 41.17 years with a standard deviation of ±8.79 and a range of 24-65 years. Of all, ninety percent patients were housewives and 52.2% belonged to upper middle socio-economic class. About 66.7% patients were pre-menopausal and 33.3% were post-menopausal. The mean BMI of respondents was 25.89(±4.67) kg/m2 and among them 51.1% were obese, 23.3% were overweight and 20.0% had normal weight. No association between BMI and vii molecular subtypes of breast cancer were noted (p>0.05). No association between BMI and hormone receptor status of breast cancer was found. Association with tumor size, axillary lymph nodes, tumor grade and lymphovascular space invasion were also not significant (p>0.05). No association between BMI and molecular subtypes, clinical and pathological features of breast cancer were noted both in pre and post-menopausal groups. This study found no association between BMI and different features of tumor, which could be attributed to small sample size, absence of control and a single centered study. However, further extensive study is recommended.

Purpose: To compare the outcome of corneal collagen crosslinking (CXL) in conjunction with antimicrobial agents (AMA) to antimicrobial agents alone in the treatment of infectious keratitis. Design: Prospective comparative clinical trial in a tertiary hospital in India. Participants: 40 patients with infectious keratitis randomised into two groups of 20 patients each- Group 1 and 2. Methods: Group 1 patients underwent CXL in addition to antimicrobial therapy. The patients in Group 2 received only antimicrobial therapy. The slit-lamp characteristics of the corneal ulceration, visual acuity, time taken for healing, and complications were documented in each group. The t-test was used for statistical analysis. P values less than 0.05 were considered statistically significant. Results: The mean time taken for healing in Group 1 was 44.5±11.2 days and in Group 2 was 27.65±7.79 days. Analysis of various parameters revealed that patients undergoing CXL showed slower healing duration (p=0.00). Conclusions: Use of CXL in conjunction with AMA lowers the risk of complications, but also slows the rate of healing of ulcers.

Covid 19 is the global pandemic that continues to surprise us day after day with its atypical clinical expressions, and its variable systemic damage from one patient to another. We report 3 clinical cases of patients with covid 19, in whom we diagnosed diabetes for the first time. The evolution of their diabetes towards remission marks its particularity. This so-called “covid” diabetes reminds us of transient neonatal diabetes.

Background: Acute kidney injury (AKI, previously known as acute renal failure) is a frequent complication that affects a substantial number of hospitalized patients annually. However, the relationship between severity of AKI and in-hospital outcomes in the setting of acute Acute ST-Elevation Myocardial Infarction (STEMI) has not been well- documented. Chronic kidney disease is a strong predictor of in-hospital mortality in the acute myocardial infarction setting. Objective: To evaluate the Outcome of admitted patients of acute ST elevation myocardial infarction with renal function. Methods: The Prospective observational study was carried out at the Cardiology Department, Bangabandhu Sheikh Mujib Medical College and Hospital, Faridpur, Bangladesh from July 2020 to June 2021. 100 patients of STEMI diagnosed by clinical, biochemical and ECG criteria were included in the study. After getting serum creatinine level from them, eGFR was calculated and patients having <60ml/min were selected for the further studies. Results: In the STEMI patients, the ratio of male patients 76% and females’ patients (35%) was 3.34:1 with mean ± SD of age was 52.84 ±8.40 year. Among the 101 patients, chart indicating the outcome of the study patients where cardiogenic shock was found in 10.8% patients, heart failure was found in 24.7%, arrhythmias were found in 69.3% and death occurred in 5.9% of cases. Table 5 showing Stratification of patients by in relation with eGFR where different pattern and frequency of outcome was observed in relation with level of eGFR described. After calculating levels of eGFR, all the outcomes showed statistically significant (P < 0.05) except cardiogenic shock which didn’t show significance (P > 0.05. Conclusion: In concluded, that those who have renal impairment previously or develop after acute STEMI have inverse outcomes. As renal impairment is associated with an increased risk of coronary artery disease and stroke, the cardiovascular disease may develop early in the course of renal dysfunction.

Background: Post tubal ligation syndrome has been a topic of debate and the association of Dysfunctional uterine bleeding (DUB) has been identified as a long-term complication. In refractory cases, hysterectomy can be considered a treatment option for managing the post-ligation syndrome due to prolonged menstrual complaints. But very few studies summarize the fact in our country's context. Considering of epidemiological data, the study was designed to assess the incidence of hysterectomy for DUB with post-tubal ligation. Methods: The hospital-based cross-sectional type of descriptive study was conducted in the inpatient Department of Gynae & Obst in Rajshahi Medical College Hospital (RMCH), From January 2019 to July 2019. Women suffering from DUB and having a history of post tubal ligation were included in the study. After selecting the patients, informed written consent was taken from the subjects. All patients were subjected to a detailed history, clinical examination, and relevant investigation. In necessary cases, hysterectomy was done as a treatment option. Data were collected using a semi-structured questionnaire designed for the study by the researcher. Data analysis and presentation were made by statistical software SPSS 23. Results: Total 100 patients with DUB were included in this study. The mean age was 40.9±6.28 SD (years), range: 25-54 years. The majority of patients (52%) belonged to the age group (41 – 50 years). About 59% came from rural areas. The mean parity was 3.43±1.34. The incidence of hysterectomy among DUB patients with a previous history of tubal ligation was 25%. The mean duration of tubal ligation was 13.4±6.3 years. The usual presentation per vaginal bleeding pattern at presentation was menorrhagia (47%), followed in second and third by metrorrhagia (20%) and postmenopausal bleeding (17%). Besides, 78% of patients also complained of tiredness, and 12% complained of pelvic pain. And Mean duration of symptoms was 13.44 months. Conclusion: About one-fourth of the tubal ligation patients underwent a hysterectomy in their subsequent life, and usually presented with menorrhagia. However, further studies are needed to finalize the incidence rate.

Introduction: Dehiscence is a partial or total separation of previously approximated wound edges, due to a failure of proper wound healing. Wound dehiscence primarily occurs after a surgery relating to an open wound. It is sometimes called wound breakdown, wound disruption, or wound separation. It can be influenced by various preoperative and postoperative factors. Various preoperative and postoperative variables might impact it, and these factors can also influence the patient's result following surgery. Aim of the Study: The aim of the study was to observe the risk factors and operative findings of abdominal wound dehiscence cases in emergency laparotomy. Methods: This prospective clinical study was conducted at the Department of Surgery, MAG Osmani Medical College, Sylhet, Bangladesh. The study duration was 8 months, from July 2008 to February 2009. A total of 460 participants were selected from those undergoing emergency laparotomy in the study place for this study. A consecutive selection method was followed for the selection of the participants. The participants were divided into two groups depending on whether wound dehiscence was developed or not. Result: All the baseline characteristics (age, body mass index, and smoking habit) of patients except sex were significantly associated with wound dehiscence. The history of receiving steroid and cytotoxic drug were higher in patients with wound dehiscence than that in patients without wound dehiscence. However, the history of receiving the immunosuppressive drug was almost identical between the groups. The patients who did not take hygienic measures or take bath before an operation or change clothing had a higher incidence of wound dehiscence. The risk factors tuberculosis, diabetes mellitus, jaundice, bronchial asthma, anemia, edema, and dehydration were found significantly higher in wound dehiscent patients compared to those without wound dehiscence. The mean duration of operation, prolene suture used for closure, midline incision, mass closure technique and drain given had significant influence on development of wound dehiscence. presence of ascitic fluid, pus and contaminated fecal material demonstrated their significant presence in patients who develop wound dehiscence than those who did not develop wound dehiscence. Postoperative conditions like abdominal distention, coughing and wound infection tend to be associated with wound dehiscence more frequently than their counterpart. Conclusion: The current study recommends evaluating patient-related risk factors before to surgery and quickly addressing them. A skilled surgeon should do surgery on high-risk patients. The study contends that the development of wound dehiscence is significantly influenced by baseline traits like age and BMI. The degree of patient cleanliness before surgery greatly influences the likelihood of wound dehiscence. According to the study, other factors that may affect the occurrence of postoperative abdominal wound dehiscence include surgical procedures, skin preparation, the length of the procedure, and the type of wound closure. Coughing, vomiting, and subsequent infection are additional postoperative side effects that increase the risk of wound dehiscence.