Bartholinitis is a common infection of the vulvar region, often polymicrobial in origin. While Escherichia coli and sexually transmitted pathogens are usually implicated, Haemophilus influenzae is rarely identified. We report a case of bartholinitis caused by H. influenzae in a young woman with no notable medical history. This case highlights the need to consider unusual pathogens in bartholinitis and the importance of an appropriate protocol for sample collection and culture.