Wheat (Triticum aestivum L.) is the most essential cereal crop among all other crops of Pakistan and belongs to the family poaceae. The experiment was performed to evaluate the response of exogenously applied jasmonic acid on wheat (Triticum aestivum L.) growth parameter against aphid attack. Two wheat varieties i.e., Fareed-2006 and Paktoon-2016 are cultivated in pots and different concentrations of Jasmonic acid (100 µM and 1 mM) were applied. Concluded results showed that aphid infested plants decreased root and shoot length, plant height, total soluble protein and chlorophyll contents but increased the POD and SOD contents, MDA contents, catalase, H2O2 concentrations and phenolics of wheat (Triticum aestivum) which was further improved through jasmonic acid application. Jasmonic acid treated plants showed better results than control and aphid infested plants. Jasmonic acid spray helped plants to recovery from aphid stress by increasing antioxidant enzyme synthesis, POD (Peroxidase), SOD (Superoxide dismutase) and CAT (Catalase). The results showed that JA sprayed plants have a reduced aphid population than controls plants. When compared to jasmonic acid (100 µM), the greater concentration of jasmonic acid (1mM) showed more effective. Use of jasmonic acid against devastating diseases seems to hold a more promising future in the pest management and agriculture sector.