Background: Cervical cancer is one of the serious diseases that affect women’s lives. Fortunately, cervical cancer is a preventable and treatable disease; early detection has reduced its mortality and morbidity worldwide. Cervical cancer screening is a global public health concern. However, there are misconceptions, stigmas, and poor awareness associated with cervical cancer. This study aimed to examine the knowledge, attitude, practice and stigmas, and misconceptions associated with cervical cancer screening in the Eastern Province of Saudi Arabia. Methodology: A cross-sectional study was conducted among the general population in the eastern province of Saudi Arabia. Data collection was carried out using a structured questionnaire that included demographic information about knowledge, attitude, practice and stigmas and misconceptions associated with cervical cancer screening. Results: The study collected data from 400 participants, with a female majority (81.8%). The majority of participants were married (65%), had an undergraduate degree (64%), and fell within the 31-50 age range (43.3%). The results showed that the majority of the participants had heard of a PAP test (61.3%), 58.8% of participants had misconceptions regarding cervical cancer screening. The association between education and knowledge of PAP tests was statistically significant (p=0.000). Conclusion: This study highlights the need for public health interventions to improve knowledge and awareness of cervical cancer screening in the Eastern province of Saudi Arabia. The study recommends public interventions targeted to concern stigmas and misconceptions associated with cervical cancer screening.