The present study was undertaken to examine the direct effect of second line anti-tuberculosis drugs Ethionamide (ETH), Para amino salicylic acid (PAS), Aloe vera on Mycobacterium tuberculosis (MTB) strain H37Rv ATCC No- 27294. It is found that Aloe vera does not interfere with single or in the combination of both ETH and PAS showing the bioenhancer activity. In vitro study of Aloe vera observed that the extract inhibited the growth of H37Rv strains. The present results will pave new avenues to find a new medicine that possesses Aloe vera alone or in combination with drugs to combat H37Rv strains controlling tuberculosis.