Aim: The present research work was planned to prepare pluronic f 127 (poloxamer 407) based hydrogel formulations of ketorolac and to evaluate the parameters like swelling behaviour, drug PH stability, invite and in vivo drug release and in vitro cytotoxic activity. Methodology: Two different strengths of ketorolac hydrogel formulations were prepared using pluronic f127 and were analysed by validated HPLC method for drug content, PH stability and in vivo drug release. Further invitro anticancer activity was evaluated using sulphorhodamine B (SRB) assay in SCC29 cell lines. Result: Both the formulations F1 and F2 showed better PH stability at PH 3.5, 5.5 and 6.8. In vitro and in vivo drug release pattern showed half life at 3 hours, AUC0−t 669 and 667ng h/ml, Cmax 884 and 872 ng/ml for F1 and F2 respectively. Conclusion: Hydrogel formulation F1 showed better percentage control growth when compared to F2 hydrogel formulation and ketorolac alone.