Background: Staphylococcus aureus is a pathogen worldwide with large disease burden. Methicillin resistant staphylococcus aureus (MRSA) is prevalent in hospital care settings and community. Timely diagnosis and treatment is essential to avert further complications of this infection as compared to Methicillin sensitive Staphylococcus aureus (MSSA). Aim of the study was to determine the prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) from pus samples in a tertiary care hospital and to analyze the antibiotic susceptibility patterns of MRSA isolates. Methodology: A cross sectional study which was done in a tertiary care hospital from Jan 2018-June 2018. Various clinical specimens were cultured and staphylococcus aureus was isolated and identified using standard biochemical tests and CLSI guidelines. Results: Out of 1090 pus samples processed 597 were growth positive and among these 196 were gram positive. Out of 196 gram positive isolates 119 were staphylococcus aureus of which 56 were MRSA & 63 were MSSA. All MRSA isolates were sensitive to Vancomycin and Linezolid and moderate sensitivity to Gentamicin and Co-trimoxazole. Conclusion: In hospital setting MRSA infection cause of worry due to resistance to commonly prescribed drugs. Regular surveillance and robust Antimicrobial stewardship can help to limit these infections.