Aim of the study was to find out the role of biochemical markers in diagnosis and prognosis of pancreatitis and compared this value with healthy subjects of Rajasthan. Total 200 subjects were selected in which 100 were selected as patients group and 100 were normal healthy subjects. Out of 100 patients 75 patients were male and 25 patients were female. patients were distributed age wise .Most of the male patients were alcohol abused And suffering from abdominal pain and most of the female patients suffering from gall stone and abdominal pain. Trypsin was estimated with sandwitch Elisa kit method and other biochemical parameters were performed by commercially available kit on fully auto analyzer. In male patients of age group 15-55 years Mean serum total and direct bilirubin level was 7.49±3.86 and 3.46±1.81 respectively. AST level was 268.22±71.74 and in control group mean AST level was 28.69± 5.85.P-value was found highly significant.(P≤ 0.0001) .as well as serum alkaline phosphatase level was found 779.86±456.77 and in control group mean ALP level in serum was 107.0±15.34 IU/L. mean value of amylase and lipase 486.86±154.20 and 2040±745.85 respectively. Mean serum level of trypsin was 461.16±200.72.in female patients all these parameters were also increased. We conclude that all biochemical parameters are increased in pancreatitis. Serum amylase and lipase both increases in pancreatitis but lipase is a specific test for alcohol induced or alcoholic pancreatitis. Trypsin can be used as a marker of pancreatitis.