А device for the isolation of nucleotide complex from biomass of Saccharomyces cerevisiae cells was developed, and the biological active substances in the extract were analyzed. The isolated nucleotide preparation can be used to stop oxidative stress and improve immunity. The developed device allows extraction of biologically active substances from Saccharomyces cerevisiae cells in series by extractants: distilled water under the action of an alternating voltage with a frequency of 105 Hz, a solution of hydrochloric acid with pH = 1.0-1.5 without using voltage. After acid extraction, the extract was neutralized with sodium hydroxide solution. The extracts obtained were combined and dried. The technical result of the device is a more complete two-fold extraction of the nucleotide complex from native Saccharomyces cerevisiae biomass. The use of our constructed device makes extraction of biologically active substances easier and complete. The content of biologically active substances, such as adenine nucleotides, amino acids, vitamins, micro-, macro- elements in the obtained extract was analyzed. A comparison of the activating effect of the isolated nucleotide preparation on Ca2+-dependent NO - synthase with T-activin was performed. Experiments showed that the nucleotide preparation, obtained by new device has a pronounced activating effect on Ca2+ -dependent NOS from rat thymocytes.