A growing body of research supports the significance of physician-nurse collaboration for the delivery of a quality patient care and positive outcomes. This study was undertaken to assess the attitudes toward collaboration between practicing physicians and nurses in three tertiary government hospitals in Riyadh, Kingdom of Saudi Arabia (KSA). A quantitative descriptive study was used to compare the difference in attitudes toward Physician-nurse collaboration between physicians and nurses. Attitudes toward collaboration as influenced by their age, gender, nationality, and number of years in practice was also explored. Data were obtained through distribution of survey questionnaire which is an adaptation of the Jefferson Scale of Attitudes Toward Physician-Nurse Collaboration (JSATPNC). Results showed a total mean scores on attitudes toward collaboration for physicians (n = 201) was 50.68 ± 8.31 and 53.59 ± 8.20 for nurses group (n=307). Mean scores were consistently higher in the 4 JSATPNC subscales of attitudes for nurses (p=0.007). Further analysis demonstrated that male physicians have statistically higher score than female physicians (p=0.043). Nurses who have more than 10 years hospital practice have higher score compared to those with 10 years and less of practice (p=0.048). Considering that physician-nurse collaboration is important in the promotion of a quality patient care and outcomes, healthcare institutions in KSA should give more focus on improving physician-nurse collaborative relationships.