The Importance of H. pylori as an etiological agent in gastroduodenal disease had suggested antibiotic treatment as a main target for the elimination of infection and to determine the prevalence of Helicobacter pylori in patients with gastro-duodenal pathologies and the susceptibility patterns of isolates Consecutive dyspeptic patients for endoscopy were recruited in the study. Gastric biopsies were collected from the patients and H. pylori isolated and identified. The present study a total six antimicrobial agents such as Ciprofloxacin, Metronidazole, Norfloxacin, Tetracyclin, Amoxycillin and Clarithromycin were used In against 100 clinical isolates Antibiotic sus-ceptibility was determined by disk diffusion and agar dilution methods against The resistance pattern, amoxicillin and metronidazole (AMRR METR) was the most common (23.7%) amongst the isolates Ninety two (83.6%) of the 110 patients (mean age 42.5 ± 15.7, range 14–70 years) were positive for H. pylori, The antibiotic susceptibility rates were 61% for tetracycline, 54.3% for clarithromycin, 16.4% for amoxicillin and 1.8% for metronidazole. Antimicrobial susceptibility results also revealed 12 antibiotypes based on resistance to the antimicrobial agents investigated.. More than 60% of the isolates exhibited multi-drug resistance to three or four antibiotics. Studies attributed the high level of resistance to the frequent use of the drugs to treat various other infections, ineffective drug control policy and the current treatment regimen in Bikaner.