Ultrasonography is a widely used imaging technique that has many clinical applications. The early staging of the metastatic carcinoma is one of these widely uses. FNA and core biopsies have exceedingly replaced the surgical biopsy. The ultrasound-guided biopsy has shown great and more accurate results for breast cancer detection more than the use of one method alone. In this study, we aimed to determine the role of ultrasound-guided biopsy in breast cancer. The procedure was performed on 32consecutive patients that were enrolled at Shaukat Khanum hospital in this Cross-sectional comparative analytical study. Ultrasound-guided core and FNA-guided biopsies were performed for all 32 patients. The accuracy of breast lumps biopsy with ultrasound guidance biopsy was more accurated as compare to non ultrasound. Out of 32 patients 1(3.1%). was diagnosedwith acute inflammation, 16(50.0%). for breast metastatic carcinoma, 2(6.3%). for chronic inflammation, 1(3.1%). for ductal epithelial carcinoma, 1(3.1%). for fibrocystic, 1(3.1%). for the fibroepithelial lesion, 1(3.1%). for lymphocytic yield, 7(21.9%). were normal results and 1(3.1%). for stromal fibrosis. In conclusion, an Ultrasound-guided biopsy is highly accurate, and more effective and feasible method than the ultrasound examination alone and excisional surgery. Recently US-guided biopsy procedure is widely used for the diagnosis of lumps in breast cancer patient.