This study on ABO and Rhesus blood group system in tuberculosis patients aimed at the assessment on prevalence of tuberculosis on human ABO and Rhesus blood group system. ABO blood and Rhesus blood group system antigens are hereditary characters that are the most clinically significant in blood transfusion. Tuberculosis is an infectious disease cause by mycobacterium tuberculosis and treated with antibiotics. The samples were collected, diagnosed and analyzed for reliable results using laboratory standard procedures, the results are represented statistically showing no significant effect(s) of tuberculosis (TB) in ABO and Rhesus blood system. This research was carried out among Igbo tribe at location of Ohaozara in Ebonyi State using two hundred (200) Tuberculosis patients coming for treatment at Tuberculosis/ Leprosy Presbyterian Joint Hospital Uburu (Happy Home) and other patient from the same location while three hundred (300) controls (people free from TB). The ABO blood group and Rhesus systems' result present blood group 0 predominated others and with 48%, followed by A (22%), B (18%) and AB (12%) while the Rhesus blood group system is 90% positive and 10% negative in Juberculosis patients. In comparism, ABO blood groups are; 0 (44%), A (28%), and AB (4%), while the Rhesus blood group system is 94% positive and 6% negative in controls. From the statistics above, among the patients in control from the same geographical location interprets that blood group O has the highest frequency of about 46 percent (46%) followed by group A with 25%, B (21%) and AB (8%) while Rhesus blood group system is 92% Rhesus positive and 8% Rhesus (D) negative in average among population study of the same location (Ohaozara). With the test (blood grouping) conducted among the population, the results show that no particular blood group is susceptible to Tuberculosis and so, Tuberculosis does not have any phynotipic effect(s) on ABO blood group and Rhesus blood group system.