A new simple, precise, accurate, sensitiveand rapid chromatographic method based on RP-HPLC was developed and validated for the estimation of Barnidipine HCl in bulk and in-house tablet dosage form. Methanol: Water (80:20 v/v)) was used as mobile phase. A gradient programing has been done, on a reverse phase C8 column (250×4.6mm×5μ) with flow rate 1 mL/min, monitored at 260 nm. The mean retention times of Barnidipine HCl were found to be 2.8 min respectively. Linearity of Barnidipine HCl was found to be 3-18 μg/mL, R2= 0.999 respectively. The developed methods have shown the best findings in terms of linearity, accuracy, precision, LOD and LOQ for API and in house tablets. The depicted method can routinely be used for the determination of Barnidipine HCl in bulk and for in-house tablets formulation