This study was conducted to determine the effect of n-butanol root extract of Leptadenia hastata and Packed cell volume (PCV) changes in albino rats. Thirty (30) albino rats comprising of both sexes were divided into six (6) groups (A - F) with each group containing five (5) rats. Group A and B were infected with Trypanosoma brucei brucei and treated with the extract at 100mg/kg and 200mg/kg respectively. Group C was infected but untreated control, while Group D was uninfected and untreated. Group E was infected with Trypanosoma brucei brucei and treated with diminazene aceturate at 3.5mg/kg. The proximate content and phytochemical properties of the extract were determined. After inoculation blood samples were collected daily to determine the parasitaemia and PCV according to standard laboratory technique. The proximate content analysis revealed the presence of dry matter, crude fiber, crude protein, moisture and ash, while alkaloids, carbohydrates, cardiac glycosides, saponin and terpenoids were the phytochemical constituents. Infected groups showed a prepatent period of 2 days with parasitaemia value increasing significantly (P>0.05) in groups (A and B) treated with extract in respective of the dose with the PCV fairly constant. The result of this study shows that the extract has no anti-trypanosomal activity but has haematinic potential