Aim: The present articles involves the investigation of anticlastogenic activity of ethanolic extract of bael fruit (Aegle marmelos). Acute toxicity study was conducted as per OECD guidelines up to 2000mg/kg body weight. Methodology: Anticlastogenic activity was investigated by two models viz, micronucleus test and chromosomal aberration method using mice. In micronucleus assay model the bone marrow was extracted form various groups of animals, staining was performed and the slides were scanned under oil immersion(100X) in LAbomed-Digi 2 microscope (90V-260V), for the presence of micronucleus in PCE (Polychromatic Erythrocytes) and NCE (Normochromatic Erythrocytes). Results: In chromosomal aberration model the animals has been sacrificed from all the groups and bone marrow was collected, processed and different types of aberrations like chromosome breaks, exchanges, rings and minute were recorded. In micronucleus assay, there was a significant (P<0.001) increase in the % MNPCE and decrease in P/N ratio in cyclophosphamide (50 mg/kg, i.p.) treated animals when compared with normal control animals. In chromosomal aberration test, there was a significant increase in total no. of chromosomal aberrations (rings, exchanges, breaks and minute) in cyclophosphamide treated animals when compared with normal control animals and it was time dependent. Bael fruit extract were found to be non mutagenic and significantly (P<0.001) decreased the cyclophosphamide induced formation of chromosomal aberrations. Conclusion: Based on the present empirical evidences the ethanolic extract of bael fruit was found significant anticlastogenic activity