Pain is the most common complaint from patients undergoing prostate biopsy. With the increase in patients requiring prostate biopsy following massive screening for prostate cancer, more men would therefore complain about pain. Observing that the perception of pain varies amongst patients, we set out to find the influence of these factors on pain experienced during prostate biopsy. We also sought the incidence of complications which may arise from this procedure. A total of 132 patients scheduled for transrectal prostate biopsy were randomly assigned to two groups. All patients had 20 mls of 2% lidocaine gel administered per rectum10 minutes before transrectal ultrasound probe insertion. The severity of pain during the procedure was assessed using the 10-point numerical rating scale. A weak positive correlation was found between the prostate volume and level of pain perceived by patients (r = 0.084) & (r = 0.339). Pre-biopsy anxiety was found to have no influence on pain. There was moderate correlation (r=0.497) between pain and age of participants during needle insertion, whereas a weak correlation(r=0.173) was found between the latter and pain perceived during probe insertion. Age was found to be the only predictor of level of pain on regression analysis (P = 0.016).Post biopsy, 25% of patients had haematuria while 12% complained of rectal bleeding. Haematospermia and fever were seen in 5.5% and 0.8% of patients respectively. Increasing age was found to influence pain perceived during transrectal ultrasound-guided prostate biopsy. Haematuria and rectal bleeding were found to be the most common complications following TRUS-guided biopsy of the prostate