Lophira lanceolata is a plant is commonly used in traditional medicine for the treatment of dermatosis, toothache, muscular tiredness and menstruel pain. This research was aimed at evaluating the in-vitro antifungal potential of different extracts and fractions of Lophira lanceolata stem-bark. The stem bark of the plant was collected, air-dried, powdered and extracted with methanol, ethanol and aqueous solvents using maceration method. The crude methanol extract (CME) was fractionated into n-hexane (HF), ethyl acetate (EF), n-Butanol (BF) and aqueous (AF) portions and were tested against pathogenic fungus strains namely; Aspergillus Niger, Candida albican, Trichophyton mentagrophyte and Trichophyton rubrum. The agar well – diffusion methods were used to determine the diameter of the zones of inhibition using 96 wells micro titer plates. The minimum inhibitory concentration and minimum fungicidal concentration were also determined. The methanol extract was found to be more effective against fungi tested, ethanol and aqueous extract which showed moderate effect against the test organisms. The methanol extract was found to be inhibitorier compared to ethanol and aqueous extracts. The results obtained revealed that, stem bark of Lophira lanceolta may contain phytochemicals that possesses antifungal activity