The wound stress is applied to leaves of P. foetida and ethyl actetate extracts is optimized using mobile phase: ethyl acetate: chloroform: glacial acetic acid: benzene (25:15:2:10) and TLC bioautographic depict two antimicrobial compounds at Rf 0.46 and 0.73. The extract shows antimicrobic activity 12 mm against S. aureus at 5 mg/ml and 14 mm against E. coli at 10 mg/ml. Their HR-LCMS chromatogram elucidates the structure of two possible antimicrobial compounds as nitrofurazone (RT: 0.644 min) or 4-tridecynoic acid (RT 7.616 min) and dihydrodeoxystreptomycin (RT:9.22min). The HPLC-DAD detected dihydrodeoxystreptomycin at 254 nm and ibuprofen at 220 nm. The GCMS confirmed 4-chloro 7-nitrobenzofurazan (RT: 18.39), methyl jasmonate (RT: 18.84), 10.03 PPM and 12-hydroxy dodecanoic acid (RT: 23.79), 82.65 PPM in 96 extract. These compound's viz 4-tridecynoic acid and dihydrodeoxystreptomycin, ibuprofen, 4-chloro 7-nitrobenzofurazan, methyl jasmonate and 12-hydroxy dodecanoic acid could be possible raised as antimicrobial drugs in pharmaceutical industries.