Stress is a stimulus or a stressful situation. If the situation is a stressful job situation, then stress on work can be caused by several factors, including workload and working conditions. The ICU nurse must monitor the patient's critical condition carefully and continuously. Hospitals are one form of health facilities, both organized by the government and society that serves to make efforts of basic health or referral health and supporting health efforts. Hospitals in carrying out their functions are expected to pay attention to social functions in providing health services to the community. ICU is one of the work environment that has high-stress tendency. It is possible since ICU nurses are exposed to patients with unsafe mental conditions, requiring particular attention for 24 hours/day. This condition requires greater physical energy with special nursing care as well. Also, critical patients demand the speed and accuracy of performing actions that are not always required in other nursing situations. Another opinion says that inadequate knowledge and skills are seen as a stress source of ICU nurses. A nurse in charge of ICU performs three main tasks, life support, monitoring the patient's condition and preventing possible complications. The type of research is observational with the quantitative approach, while the research design used is cross-sectional that is by examining the dependent variable, free and variable between simultaneously. With a sample of nurses 11 people. Data analysis method by univariate analysis and bivariate analysis. The workload of nurses who work in the ICU area of the majority "Medium" are five people (45.5%). The working conditions of nurses working in ICU areas of the majority "Medium and Hard" are four people (36.4%). The stress levels the work of nurses working in the ICU area of the majority "Medium" are five people (45.5%). There is Workload Relation with Stress Level of Respondent in ICU is p <0,05 (p = 0.032), no Relation of Working Condition with Stress Level Respondent in ICU Year 2017 is p> 0.05 (p = 0.775). The hospital has to give counseling, education and training of emergency and the use of supporting tools available in ICU room so that they can anticipate the obstacles faced during the working period.