Chronic supportive otitis media is highly prevalent in the developing countries like India. The present study was conducted for duration of three years in two Medical Colleges of West Bengal, India, to find the prevalence of bacterial infections in otorrhoea. Chronic suppurative otitis media was more common in the rural population (66.8%) than in the urban population (33.2%). Out of the 226 patients in the study, 144 cases (63.7%) of purely bacterial, 59 cases (26.1%) of mixed (bacterial and fungal), and 23 cases (10.2%) of purely fungal infections were isolated. Out of the total 226 cases of chronic suppurative otitis media, the number of bacterial infections was 203 (89.8%). The most common age group observed was 1-10 years (32.3%). There was no significant difference between male and female preponderance, with a male, female ratio of 1.17:1. Pseudomonas aeruginosa was found in 87 (38.50%) cases and was the most common organism, followed by Staphylococcus aureus in 51 (22.57%) cases. Other bacteria found in our study were Klebsiella species in 20 patients (8.85%), Enterococcus species in 18 patients (7.96%), and Proteus species in 16 patients (7.07%), Citrobacter species in 9 patients (3.98%) and Serratia marcescens in 2 patients (0.88%). We found that antibiotics effective against most of the organisms were gentamicin, followed by ciprofloxacin. Coamoxyclav was found to be effective mainly against Staphylococcus aureus (94.1%) but ineffective against the Gram negative bacteria. Ciprofloxacin is recommended as a first line antimicrobial for chronic suppurative otitis media, as it has no ototoxicity, has lower cost and is available both in topical and oral preparations