A colorimetric method was used for the determination of vitamin K1 phylloquinone in different plant extracts (Alchornea cordifolia, Aspilia Africana, Chromolaena odorata, Vernonia amygdalina). Benzene was used to extract the plants after drying and milling. The method of vitamin k determnation utilizes the coupling of 2,4-dinitrophenylhydrazine, in which a hydrazone of vitamin K, forms acid-salts in basic media giving blue coloured solutions that absorbs maximally at 635 nm. The results showed that Aspilia Africana 2.43 ± 1.56, Vernonia amygdalina 2.12 ± 1.11, Chromolaena odorata 6.36 ± 2.09, Alchornea cordifolia 8.31 ± 1.23 ug/g dry extract