A simple and sensitive spectrofluorimetric method has been developed for the estimation of Gefitinib in pure and pharmaceutical dosage form. Gefitinib exhibits maximum fluorescence intensity in ethanol and Beer’s law was obeyed in the range of 1-3.5 µg/mL at an excitation wavelength (λex) of 280 nm and an emission wavelength (λem) of 512 nm. Stability studies with respect to time and temperature were also carried out. The results obtained were in good agreement with the labeled amounts of the marketed formulations. This method has been statistically evaluated and found to be accurate and precise