Blood transfusion system is a basic necessity of health care delivery system. As off now more than 700 antigens and about 30 blood group systems have been identified. ABO and Rhesus (Rh) blood group systems are most important tool for blood transfusion, parental testing, legal medicine and in population genetic study. It is immensely important to identify the working pattern of a blood bank of rural West Bengal as it will facilitate to generate appropriate policies for better service. The main objective of the study is to determine age and sex wise frequency and distribution of ABO and Rh blood group pattern among blood donors and working pattern of newly developed blood bank and compare with other data from similar studies. Retrospective study was conducted at Blood bank of Khatra Sub-divisional Hospital, West Bengal over a period of one year. Blood group of the donors was determined by standard monoclonal antisera test by tube agglutination technique. Out of 1802 blood donors, 88.62% were male and 11.38% were female. 63 donors were deferred due to hypertension and diabetes. The commonest blood group observed was O+Ve followed by B+Ve and the rare one was AB-Ve. The study had a significant implication regarding the inventory management of blood bank and transfusion services for the patient admitted in the sub-divisional hospital of rural West Bengal and also necessary for the health planners while making efforts to face the future health challenges in this region