In present study an attempt has been made to study the antimicrobial activity and chemical composition of leaves and flowers of Spilanthes acmella. The leaves and flowers of the plant were collected and subjected for study of their chemical composites and antimicrobial activity of their extracts. The samples were given the code SALS1, SALS2 and SALS3 and SAFS1, SAFS2 and SAFS3 respectively for the extracts of leaves and flowers. The crude extracts of leaves and flowers were prepared by using various solvents like Petroleum ether, Ethanol and double distilled water. All the extracts were used to study their antimicrobial activity against gram positive bacteria eg. Bacillus subtilis, gram negative bacteria eg. E. coli and K. Pneumonia and anti-fungal activity against Aspergillus Niger. The chemical composition of leaves and flowers like Dry matter, Total ash, Ether extract, Crude fibre, nitrogen, Crude protein, Total carbohydrates, Nitrogen free extract and Organic matter were studied. The results of antimicrobial activity revealed that all the crude extract samples of leaves were found to possess antibacterial property. Maximum inhibition was shown by all the three samples against E. Coli and K. Pneumonia. However the maximum inhibition of growth of E. Coli and Bacillus subtilis was shown by SAFS1 and SAFS2 respectively. All the crude extracts of samples shown antifungal activity against Aspergillus Niger. The samples of leaves were found to be more effective than flowers against the fungal strains of Aspergillus Niger. In present study it has been concluded that the leaves may be used against the infectious diseases caused by E. Coli, K. Pneumonia and A. Niger as herbal medicine