Single Cell Protein (SCP) can be obtained from various microorganisms by growing them on a number of types of substrates, including industrial products waste. This work focuses on non-conventional yeast Yarrowia lipolytica, and its application in the process of production of valuable components like SCP and amino acids. It also analyses the possibility of using obtained biomass as a dietary supplement for humans. Y. lipolytica is nonpathogenic to humans and has been approved for use in several industrial processes as it is generally recognized as safe (GRAS). The results of our analyses suggest that the Y. lipolytica A-101 – a strain growing in biofuel production waste is a good candidate for a source of high-quality yeast protein (40-50% of dried biomass) and exogenous amino acids (phenylalanine 3.9 g, isoleucine 4.4 g, leucine 6.8 g, lysine 7.0 g, methionine 1.2 g, threonine 4.8 g, tryptophan 4.7 g, valine 5.3 g/100 g of protein). Y. lipolytica A-101 biomass has also very low nucleic acids concentration (below 1%) due to activation of endogenous nucleases in the final stage of the stationary phase. These enzymes reduce the amount of nucleic acids in this biomass from a high level to one acceptable for human consumption. Moreover, Y. lipolytica A-101 biomass is safe and digestible since the yeast cells get killed in the drying process and has no leavening powder with destroyed cell wall. Y. lipolytica A-101 can be used as a nutritional supplement in human diet when an increased intake of amino-acids and SCP is required.