An attempt was made to validate Sterility Assurance level (SAL) of two Industrial Autoclaves up to 106 Log Reduction by using both gram negative and gram positive bacteria. For gram negative bacteria, a suspension of E. coli was prepared at 108 cfu/ml which was sterilized individually through two autoclaves at 1210C for 20 minutes at three consecutive cycles. Bacterial load was then determined and found 8 log reductions of bacterial growth. For Gram positive bacteria, 12 ampoules of Geobacillus stearothermophilus containing 106 cfu were placed in two Autoclaves. After sterilization, no growth of Geobacillus stearothermophilus was found which also proving the 106 Log Reductions of that sterilization parameters