Gluconeogenesis involves formation of glucose from the breakdown of protein to form glycogenic amino acid and lipid such as triglyceride to generate glycerol which is further utilized for the formation of glucose. Cashew (Anacardium occidentale) leaf has some non-scientific but traditional health benefit claims such as in the treatment of diabetes and hypertension. This work was designed to determine the evidence of Gluconeogenesis in rabbits given ypung cashew leaf extract using Plasma Value of Cortisol, Glycerol, Cortisol Binding Globulin Glucose, Total Triglyceride and Total Bile Acid. Materials and Methods: 10 rabbits of the same sex weighing 1.0-1.2Kg grouped into A1 (5 rabbits fed with normal meal and water only for 7 days), A2 (A1 rabbits given 500mg/KgBW of ethanolic extract for another seven days) and B1 (5 rabbits fed with normal meal and water only for 7 days), B2 (A1 rabbits given 500mg/KgBW of aqueous extract for another seven days). Plasma cortisol, glycerol, cortisol binding globulin, glucose, total triglyceride and total bile acid were measured biochemically using auto-analysis, ELISA and colorimetric techniques. The result obtained showed a significantly higher plasma value of cortisol, Glycerol and a significantly lower mean plasma value of Glucose and Total Triglyceride in rabbits (A2 and B2) given 500mg/KgBW of ethanolic or aqueous extract for seven days compared with the values of these parameters obtained in the same rabbits when they were fed with normal meal and water only for 7 days (A1 and B1) with p<0.05. This work reviled possible evidence of gluconeogenesis as indicated by the significant biochemical alterations the plasma values of cortisol, glycerol, glucose and total triglyceride with respect to changes in their plasma level before and after the supplementation of the extract of young cashew (Anacardium occidentale) leaf