Catechin and quercetin are the major bioactive phenolic compound of Acacia catechu, was estimated and validated by RP- HPLC in khadirarista. The analytical method validation was carried out according to ICH method validation guidelines. The development was carried out in Lichrosper 100 C-18(250*4.6*5) column and gradient elution. The determining wavelength was confirmed as 280nm and 360nm for catechin and quercetin. The detection and quantification limits of catechin were 0.57mcg/ml & 1.74mcg/ml and for quercetin 0.52mcg/ml & 1.51mcg/ml respectively. The developed HPLC method is very accurate, precise and cost effective and it can be successfully applied to the assay of marketed formulation