Employing standard precautions means taking precautions with everybody. If precautions are taken with everyone, health care workers do not have to make assumptions about people's lifestyles and risk of infection. Health care workers should have the right to protect themselves against infection, whether it is human immunodeficiency virus (HIV), Hepatitis or anything else. And clients have also the right to get safe service. The main objective of the study is to assess the knowledge, attitude and practices of Nurses on Standard precautions. The quantitative cross-sectional study design was used with random sampling (n=150) from Mayo Hospital. Using the self-administered questioner, data was analyzed SPSS 21 version and application of chi-square test with (p=<0.05). Current study showed that participant always wash hands immediately after removing gloves were 92 %( n=139) agree, nurses should take self-protective measure, 75.3% (n=114) participants were agree. Participant who don’t always wash hands after removing protective gloves were (n=99)92.0% participants were agree. AIDS presents a serious occupational hazard to health care work 94% (n=144) Participants were agree. There was no significant association found in qualification and knowledge of nurses regarding recapping of infected needle, by recapping infected needle risk of AIDS is decrease .The qualification of participants did effect on the recapping of infected needle p=0.083 with chi-square value a which is >.05.Significant association in qualification and transmission of disease by infected needle. Every nurse should have a knowledge attitude and practice of standard precaution. There is a need to improve the knowledge and provide training especially universal precautions transmission of disease