It is important for any health system to provide Nursing care and treatment with great quality and respond the client’s necessities. About Hepatitis C disease discrimination and stigmatization is very common in health care sector. In India nursing college student stated that Patients with hepatitis C often encounter incrimination and prejudice in the hospitals, by the family and common people. Because of increasing prevalence of Hepatitis C, It is very important to take appropriate action regarding HCV in Government Hospital of Jhang. The aim was to assess the exact knowledge and attitude of Nurses about care and treatment of Hepatitis C. Quantitative descriptive cross sectional study design was used and questioners on five point Likert scale ranging from strongly disagree, disagree, neutral, agree, strongly agree were distributed . Simple convenient sampling technique was used and Sample size was 222 Nurses from selected hospital. Reliability was checked on Cronbach, s alpha, it was .886 for knowledge and .738 for attitude that is reliable. Data was analyzed SPSS software 21. Most of the participant shown negative attitude regarding HCV 65% (n=146) shown sorry for HCV contracted through blood transfusion. 72% (n=160) respondent not like treating patient with HCV. 69.8% (n=155) respondent were not willing to treat HCV patient. 72.4% (n=162) not believe that their profession plays an important role in HCV treatment. 83.7% (n=186) agree that that they do, not have skills needed to effectively and safely in treating HCV patient .There was association between knowledge and attitude score. Study result shows lack of readiness about HCV patient, that is due to bias and stigmatization .It is therefore very important to improve Nurses knowledge and attitude about hepatitis C disease.