Epidemiological information on tuberculosis (TB) has always been vital for planning control strategies. It has now gained further importance for monitoring the impact of interventions to control the disease. The present study was done to estimate the prevalence of pulmonary tuberculosis in industrial population in a tertiary care centre in north India. It is a cross sectional study conducted at ESIC Medical college and hospital which caters to the registered /insured industrial worker under ESI scheme. All patients from chest clinic in the study period who were presumptive pulmonary TB patients were subjected to two sputum examination for AFB microscopy by ZN staining method. Demographic data of study subjects was collected, processed and statistical analyzed using SPSS software. Total 1315 patients participated in the study. Total sputum samples collected were 2623 out of which 351 were positive giving slide positivity rate of 13.58%. Among all of the total positive slides, male female ratio was 1.4:1. Maximum number of slide positive cases was present in 21-30 yr of age group in both male and female. 15.2% cases observed one of the two sputum samples as positive, thus emphasizing the importance of at least two sputum samples for diagnosis. The findings of this study reveal that younger age group patients 21-30 year old were most susceptible to tuberculosis and also percentage positivity of sputum among females was found significantly higher than males in the reproductive age group (21-50 yr). This finding, if corroborated in further studies could have major implications on the reproductive health of women