Time out protocol for surgery is very important for the declination of mortality rate because two hundred and thirty four millions surgeries have been performed every day worldwide in which 7 million had adverse complications. To assess the attitude and practice of surgical team members regarding time out protocol before surgery in Operation Theater. A descriptive, quantitative cross-sectional study design was done. An adopted questionnaire have been distributed among 154 participants to collect data from Services Hospital Lahore. SPSS version 21 was used to analyze the data. Descriptive statistics and chi-square were applied. In this study out of (N= 154) respondents 50 %(n=77) of the participants were male and 50%(n=154) were females and 58..4%of the respondents were between the age of 26-30 years.45% of the surgeons and 27% of anesthetists and only 26%of the nurses were participate in the study.50% of the participants were 6- 10 year of job experience. Respondents towards the attitude of time out protocol were positive.87%of the respondents think that check the patient identity, procedure side is a joint responsibility.92% of respondent said that time out protocol can avoid incorrect surgery.76%of the respondent confirm the patient identity before every operation.81% of the respondents confirm that surgical procedure before each operation. The Time out protocol is one of the important strategies to minimize the hazardous situation in Operation Theater. It is important in Operation Theater and surgical ward as well as in overall hospital to identify the patient properly, his diagnosis, his treatment planning, his medication and the site or part of surgery to be carried out