In the health sector, there is an emergent attention in spiritual care, which has resulted in more research around the topic spirituality and spiritual care. But, here is little research showing how nurse’s provide spiritual care. Nursing care of quality recognized by patient supporting and enhance overall patients spiritual needs. In nursing profession used skills for patient care as a set of spiritual care. The therapeutic relationship between nurses and patients sign of spirituality. Nurses responsible to listen actively, show empathy and hope and provide all facilities for patients. Aim of this study, “to assess spirituality and spiritual care in nursing practice in public hospitals Pakistan”. Descriptive cross-sectional survey was conducted in 2017 from nurses at work in public hospital, Pakistan. Data were collected using the spirituality and spiritual care related questions. Total 251 female nurses’ participants completed the survey questionnaire. Questionnaire were completed by 100% (n=251) of the intended convenience sample. Data was analyzed using SSPS 0.21. Descriptive inferential statistics, chi-square, correlation and regression were used for the study. Significant association found among spirituality and spiritual care, the average score spirituality 50.6% and spiritual care was, 41.4% result indicating a fair score. Education is warranted to improve nurses ‘more consciousness related to patient spiritual needs. Spiritual education considerate these needs that provide to nurses with these opportunities to address spirituality and spiritual care that reflect the nurses- patient rapport.