Standard isolation precaution is a way to stop the spread of hospital acquired infection which may be in the form of blood, secretions, body waste, body fluids and mucous membrane that may contain contagious infectious agents. Nurses are the persons who have the moral obligation to care for sick persons and improve their regaining health and attain excellent worth of treatment care. Therefore health care providers should have proper awareness and good practice to strictly adhere to standard isolation precaution to infection control. The aim of the study is to assess the knowledge, attitude and practice of nurses toward standard isolation precaution among registered nurses. Study design of this study descriptive cross sectional was used and 132 participants were participate in this study from Children Hospital, Lahore through convenient sample method and all of the participants were female nurses. Results of the study revealed that answers from the participant clearly predicted that most of the nurses have good knowledge and unsatisfactory attitude and practice toward standard isolation precaution. Conclusion of the current study concluded that using standard isolation precaution is very important for nurses to control hospital acquired infection. Study finding also show that most of the nurses contributing in the study have good knowledge about standard isolation precaution but there attitude were not satisfactory toward standard isolation precaution. Study finding also predict that most of the nurses also have unsatisfactory practice and they do not use standard isolation precaution to control infection