Pressure ulcer lead to pain and discomfort for patients and also cause prolong illness, delay restoration, increase patient’s hospital stay, and may lead to infirmity and even death. The purpose of this study was to assess the nurse’s knowledge and practices towards pressure ulcer prevention. To check the association between nurses job experience and their knowledge and practices towards pressure ulcer prevention at Lahore General Hospital. A cross sectional study among 253 nurses conducted in Lahore General Hospital. The study participants were selected by convenience sampling technique. An adopted questionnaire distribute among study participants. Data entered and analyzed in SPSS version 21 for descriptive statistics and results presented in Graphs and Tables. Chi-Square test used to check the association between nurses job experience and their knowledge and practices. Nurses have poor knowledge (35.2%) regarding risk factors of pressure ulcer development, patient’s position every two hourly (49%), knowledge about the use of the risk assessment scale fore pressure ulcers was (37.2%), in order to give pressure ulcer prevention education to patient’s and caregivers(36%). Nurses overall knowledge was only (8.3%) have good knowledge, (11.1%) have fair knowledge and (80.6%) have poor knowledge about pressure ulcer prevention (82.6%) have poor practices, (7.1%) have fair practices and (10.3%) have good practices of pressure ulcer prevention. Nurse’s job experience has a significant association with their practices and knowledge toward pressure ulcer prevention. Nurses overall knowledge and practices toward pressure ulcer are poor. Working experience had a significant association with nurses Practices and knowledge at Lahore general hospital. So nurses need to get continuing education and training about pressure ulcer prevention that will enhance their knowledge and practices can be improved