Hand hygiene is a primary measure to reduce the infection in neonates and reduces the rate of morbidity and mortality rate. Contrary to this, negation of the hand hygiene compliance among health care workers increases the morbidity and mortality rates in newborn babies. The purpose of this study is to determine hand hygiene compliances among the nurses about caring newborn babies among the government hospitals of Pakistan. The self-administered questionnaire was distributed to 110 nurses of public hospitals of Lahore, Pakistan through convenient sampling technique. The results of the study show that nurses of public hospitals have low to moderate perception regarding the benefits of the hand washing. Similarly, the nurses of public hospitals of Lahore, Pakistan perceived difficult to extremely difficult to follow the protocols of washing hands. Therefore, public hospital’s management should emphasize on the education, skills development and training of the nurses regarding the hand washing protocols and make them accountable for the ignorance