Clinical practice guideline having much importance to improve the patient care outcomes and safety from complications. Nurses depend on multi-disciplinary knowledge and skills to use of clinical practice guidelines to promote their nursing practices. Nursing staff adherence to clinical practices guidelines is help in improving patient safety in hospital. The purpose of study is exploring the factors that promotes and inhibit adherence to clinical practices. This study considers the public hospital due to poor health care services. The population is comprised of registered nurses who are working in Jinnah hospital Lahore is included except critical areas, head nurses and other health care professionals. Sample size consists of 175 registered nurses of Jinnah hospital. According to analysis by using SPSS 21, results show that the organizational factors like development of guidelines, resources to carry out the skills, and support from hospital administration are the major promoters to adhere the clinical practice guidelines. Moreover, the results of this study depict that the personal factors including nurses have not enough knowledge about clinical practice guidelines, leadership and peer support, skill development, lack of communication, lack of resources and other facilities are major barriers to adherence the clinical practice guidelines. Finally, as a result of the research, multifaceted recommendation to assist the policy makers of health care organization and nursing managers to updating the guidelines, conduct educational and training session for nurses in a manner to up gradation of knowledge and improve the overall care and safety of patients