The current study was planned to evaluate the phytochemical analysis and oral acute toxicity study of the leaves of Baccaurea ramiflora and Microcos paniculata. The soxhlated extraction and standard methods were used for extractions and phytochemical analysis. The drug was administration orally, animal’s observed from cage side observations and mean body weight was taken for 14 days. There phytochemical analysis of ethanolic extract of the leaves of Baccaurea ramiflora and Microcos paniculata revealed the presence of alkaloids, carbohydrates, glycosides, tannins, phytosterols, saponins and flavonoids. The oral acute toxicity study showed no noticeable clinical signs of toxicity and mortality during 14 days of the study period. No statistically significant alterations in body weights, compared to control group. We conclude that the outcome of the present study may add scientific information of ethanolic, chloroform extracts and acute toxicity study of the leaves of Baccaurea ramiflora and Microcos paniculata to the system of medical specialization. Ethanolic extract of leaves of Baccaurea ramiflora and Microcos paniculata was found to be nontoxic up to 5000 mg/kg body weight, so the LD50 is greater than 5000 mg/kg body weight