This study measured the blood pressure, lipid, lipoproteins and total antioxidant status in physically active premenopausal and postmenopausal school teachers in selected secondary schools in Osogbo, Osun State, Nigeria. Thirty six premenopausal (group I) and forty postmenopausal (group II) participated in the study. Body weight, height, waist- hip circumference, Systolic blood pressure (SBP) and diastolic blood pressure (DBP) of the teachers were measured. Body mass index (BMI), waist-hip ratio (WHR), pulse pressure (PP) and mean arterial blood pressure (MAP) were calculated using standard formulae. Total Cholesterol (TC), Triglycerides (TG), high density lipoprotein cholesterol (HDL-C) were measured by enzymatic methods. Low Density Lipoprotein Cholesterol (LDL-C) and Very Low Density Lipoproteins Cholesterol (VLDLC) were calculated using Friedewald's equation. Total Antioxidant Status (TAS) was measured by spectrophotometric method. Results showed that the means of BMI (30.98 ± 4.86), WHR (0.87 ± 0.10), TG (1.74 ± 0.50) and VLDL (0.79 ± 0.23) in group II were increased (p<0.05) when compared with corresponding means of BMI (28.19± 4.81), WHR (0.85± 0.06), TG (0.89 ± 0.80) and VLDL (0.40 ± 0.36) in group I. A decrease in mean TAS (1.16 ± 0.07) in group II was observed when compared with corresponding mean TAS (1.24 ± 0.03) of group I. A negative association (r = -0.371, p<0.01) was observed between age and TAS. No difference was seen in mean SBP, DBP and levels of TC, HDL-C, and LDL-C in group II when compared with values of group I. Changes in TAS, lipids and lipoproteins observed in post menopausal women could be associated with oxidative stress, aging process and hormonal changes in menopause and could lead to an increased risk in developing CVD despite absence of a sedentary lifestyle.