In the present study, a series of N-[6-fluoro-7-substituted-1,3-benzothiazol-2-yl]-4-[2-(3-nitrophenyl)-4-oxo-thiazolidin-3-yl]benzenesulphonamide and N-[6-fluoro-7-substituted-1,3-benzothiazol-2-yl]-4-[2-(4-nitrophenyl)-4-oxo-thiazolidin-3-yl]benzenesulphonamide derivatives were synthesized in good yields and characterized by FTIR, 1HNMR, mass spectral and elemental analyses. All synthesized compounds were evaluated for their preliminary in vitro anti-mycobacterial activity against Mycobacterium tuberculosis H37 Rv strain by tube dilution assay method. Some selected synthesized compounds were also evaluated for cytostatic activities against malignant human cell lines: cervical (HeLa), bone marrow (K-562) and kidney cell (HEK-293)