An attempt was made in the present study to test for in vivo Brine Shrimp Lethality Assay (BSLT) and molecular docking studies of some newly synthesized 2-substituted 4,6 di chloro 1,3 triazine-chalcone hybrids as potential cytotoxic gents. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Thirty nauplii were added into three replicates of each concentration of the synthesized compounds. After 24 hours the surviving brine shrimp larvae were counted and LC50 was assessed. Schrodinger Mastero docker was used for insilico molecular docking studies on selected cancer protein Targets. The results drawn from the preliminary studies showed that the compounds TCH-9, 12 and 20, 24 were potent against the brine shrimp with minimum LC50 values of 3.125, and 6.25μg/mL respectively. These findings suggest that the synthesized 1,3,5 triazine chalcone hybrids possess potential cytotoxic activity.