In situ gel is a novel ophthalmic dosage form, which persists as solution form at room temperature but undergoes gel transformation when instilled into ophthalmic cavity, thus enhancing the patient compliance. In this present study ion activated in situ ophthalmic gel of Moxifloxacin hydrochloride and Ketorolac tromethamine combination are prepared by using gelrite (0.1-0.25% w/v) as gelling agent and carboxy methyl tamarind kernel powder(0.2-0.45% w/v) as viscosity enhancing agent and rate controlling polymer, Benzalkonium chloride in suitable proportion was used as preservative. The compatability study between drug and polymer was done by FTIR and DSC. The formulations were sterilized by autoclaving at 1210 C at 15 PSI at 20 min. The formulations passed various evaluation parameters like visual appearance, clarity, pH, gelling capacity, drug content and in vitro diffusion studies, antimicrobial study, stability study. The drug release pattern was analysed by derivative spectroscopy using ZCP method. The release pattern of the best formulation indicated that both the drug showed sustained release pattern for a period of 12 hours thus enhancing the contact time of drug with ocular tissues and reducing the nasolacrimal drainage. From this present work it can be concluded that In situ ophthalmic gel is an alternative to convention drug delivery system