The aim of this study was to evaluate the antinociceptive and neuropharmacological activities of methanol extract of Nymphaea nouchali (Nymphaeaceae) flower (MENN).The antinociceptive activity of MENN was evaluated by heat induced (tail immersion test) and chemical induced pain models (acetic acid-induced writhing). The effect of MENN on central nervous system (CNS) was studied using hole cross test, open field test. MENN showed strong, significant and dose-dependent antinociceptive activity in both acetic acid-induced writhing and tail immersion test a tall experimental doses (200mg/kg and 400mg/kg). Acetic acid induced writhing test revealed that the extract at the lower dose inhibited59.97% and at the higher dose produced a maximum of 64.75% inhibition of writhing that is comparable to the reference drug Diclofenac Sodium. MENN also showed reduced locomotor activity in both hole cross and both open field tests. So, it is evident that MENN possesse strong antinociceptive activity as well as CNS depressant activity. The results justify the ethnomedicinal use of N. nouchali flower in different painful conditions and CNS disorders