Radiological accident can have a huge impact on public health. Emergency physicians should be prepared to respond to potential radiation emergencies by improving their knowledge and attitude regarding radiation emergency response. This study was consisted of a cross sectional survey that conducted among Emergency physicians in Aseer region, southwest of Saudi Arabia by 2015. The questionnaires were distributed during annual emergency physicians meeting in Aseer region of Saudi Arabia and collected in the same event. Data were coded and enter to computer provided with statistical package for social sciences (SPSS 15). The survey response rate was 88%. Only 10.3% of respondents had attended radiation emergency training course. Those who attended training courses and with poor knowledge for utilizing PPE, decontamination, diagnosis and utilizing chelating agents 40%, 40%, 30% and 40% respectively. And those who were not attend training courses and with poor knowledge for utilizing PPE, decontamination, diagnosis and utilizing chelating agents 71.3% ,72.4%,65.5% and 80.5% respectively. 40% of those who attended training courses unlikely to come to work while 44.8% among those who were not attending training courses unlikely to come to work. 40% of attending training courses respondents feels uncomfortable to take care of victims and 30% of them feel uncomfortable to do decontamination for victims. 46% of those who were not attending training courses feel uncomfortable to deal with victims and 42.5 % of them feel uncomfortable to do decontamination for victims. Low level of knowledge and attitude among most of emergency physicians in Aseer region was discovered. Training courses, which were provided improving emergency physicians knowledge but doesn’t change their attitude toward attending and caring of victims.