The effect of myeloid leukemia therapy has been widely noticed especially in acute myeloid leukemia mainly of liver enzymes. The objective of this study was to estimate the concentrations of some liver biochemical parameters (enzymes and proteins) including total protein, albumin, globulins, AST, ALT, ALP, and GGT, as well as to study correlation between these parameters in myeloid leukemia patients. This is a cross-sectionalstudy conducted in Institute of Nuclear Medicine and Oncology in Wad Madani City, Gezira Stateduringthe period from October to November 2015. The blood samples were obtained from 110 patients, 60 newly diagnosed myeloid leukemia with nottreated yet, and 50 patients treated with chemotherapy, all biochemical parameters were assessed using reagent kits, spectrophotometric method (BioSystem, Spain-Bercelona) was used. The mean of AST, ALT, GGT showed statistical significant increase, p. values (0.01,0.005, 0.026, 0.04), respectively compared to reference values.Mean levels of AST, ALT were higher in pretreatment <20years when compared with post-treatment <20 years, p.values (0.008, 0.025), respectively, as well as in pre and post treatment >20 years group also they observed significance values (0.000, 0.001), respectively. ALP enzyme level was significantly elevated in only the post treatment >20 years group, p.value (0.03). Pearson's correlationsbetween (age/disease, AST/ALT, AST/ALP, ALT/ALP) of the pretreatment group was found significant positiver. values (0.438, 0.886, 0.540, 0.492) p.value (0.000), while in post treatment group r.values were (0.272, 0.511, 0.960, 0.512), respectively and p.value for each (0.000). This study revealed that, thechemotherapy of myeloid leukemia in patients causes significant change in serum liver enzymes AST, ALT and GGT. There was a positive correlation between the myeloid leukemia disease and elevation of liver enzymes of pre and post treatment patients groups