Anabolic Androgenic Steroids (AAS) are being used widely by athletes and gym attendees to augment their physical appearance and enhance performance. The abuse of AAS has serious and irreversible health risks. Objective: To estimate the prevalence of AAS use, assess the knowledge, attitude, practice and describe the associated factors of AAS use to improve the general health of adult male fitness centres attendees. Design: Cross Sectional Study. Setting: Fitness centres in Bahrain. Method: A total of 235 fitness centres were obtained and only 94 fitness centres met the inclusion criteria. Adult males aged 18-65 years old who attended fitness centres during October 2017 were targeted. Self-administered questionnaires were distributed among 20 randomly selected fitness centres covering the 4 governorates. Result: Four hundred forty six males were included. The prevalence of AAS use was 14.6%, the highest result was 26.4% found in Muharraq governorate. The majority of AAS users were Bahrainis (17.8%) and aged 30–39 years old (17.9%). Both AAS users and non-users had low knowledge score. Regarding the harmful effects of AAS, 18% of AAS users thought AAS use is bad for health. The most common route of AAS use was the combination of injectable and oral route (63.5%). Significant association was found between AAS use and the use of other supplements such as growth hormones, proteins and amino acid, smoking and energy drink consumption (P=0.05)