Chronic Kidney Disease (CKD) is a progressive diminution in renal function especially in normal excretory and regulatory functions of the kidney. Kidney function is assessed in clinical practice to screen for kidney diseases. It is of prime importance to evaluate kidney function as accurately as possible due to different clinical presentations during the cource of the disease which are often asymptomatic. Evaluation of biochemical markers like serum BUN, creatinine, BUN:Cr ratio and ALP could play an important role in accurate diagnosis and in assessing risk of renal failure that could assists in adopting therapeutic strategies to minimize the mortality rates associated with renal failure. Keeping in view, one hundred patients with varying degree of renal disease categorized as mild, moderate and End stage renal disease (ESRD) were enrolled in the present study. Serum levels of BUN, creatinine, uric acid and ALP were measured and analysed statistically by SPSS software. Males had higher levels of BUN, Creatinine, uric acid compared to females. Serum levels of these markers were found to increase significantly (P<0.05) from mild to moderate CKD to ESRD patients. Thus, indicating role of BUN:Cr ratio as efficient prognostic marker in diagnosis of renal failure. Also pre-dialysis and post-dialysis evaluation of these markers done in ESRD patients resulted in significant decrease in these markers following dialysis. Hence, confirming dialysis as an efficient renal replacement therapy in patients with ESRD