This study was conducted to determine the association of Insertion-deletion Polymorphism in the Angiotensin-Converting Enzyme (ACE) Gene and Human Essential Hypertension among Sudanese in Al Nubba Village. A case-control community-based study was performed, Blood samples were collected from 54 subjects in a rural area around Khartoum (Al Nubba Village). The frequencies of the insertion (*I) and deletion (*D) alleles of the ACE gene were investigated, Polymerase chain reaction (PCR) was detected for the Insertion/Deletion polymorphism. Allele frequencies were calculated, our data indicated a preponderance of the *D allele among the Sudanese population. The percentage of Case/Control for Homozygous deletion DD, Homozygous insertion II and Heterozygous DI are almost the same. Further studies are recommended with a large sample size