Background: The retrohepatic segment of the inferior venacava lies in the groove on the posterior surface between caudate and the right lobe of the liver and has the hepatic veins opening into it, these openings are known as “Ostia Venae Hepaticae”. Aims & objectives: The aim of the present study is to analyse and interpret the axial direction course and its variations in the retrohepatic segment of inferior venacava in the Tamilnadu population. Material and Methodology: Seventy formalin-fixed adult liver specimens were taken for this study and carried out in the department of anatomy, SRMC &RI, during the year 2012-2015. To keep the liver in anatomical position to find out the axial direction in the retrohepatic segment of the inferior venacaval groove concerning the longitudinal axis of the liver, it was observed to have vertical, oblique toward left or curved toward left. Observation & Results: Our observation of the oblique axial direction of the RHIVC was close to the other observations reported by previous authors in Indians. The incidence of the left curve in the present study was close to the results reported by the majority of previous authors. The vertical orientation of RHIVC was similar to the results reported in the existing literature. In the present study in Tamilnadu population n=70, axial direction shows n=18-25.7%, vertical n=32 - 45.7%, oblique n=20 -28.5%. Conclusion: To conclude, this study re-emphasizes the vitality of the knowledge of the varied pattern of the retrohepatic segment of inferior vena cava in facilitating an accurate and better-designed road map for the hepatologists, liver transplant surgeons and interventional radiologists.